NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66980

Recombinant Monoclonal Antibody
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NBP2-66980
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM59-11 expressed in HEK293

Concentration

1 mg/ml

View all NGFI-B alpha/Nur77/NR4A1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human NGFI-B alpha/Nur77/NR4A1 aa 10-49 / 598. (SwissProt: P22736 Human; SwissProt: P12813 Mouse; SwissProt: P22829 Rat)

Localization

Cytoplasm. Nucleus. Nucleus membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11)

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of NUR77 on rat brain tissue lysates using anti-NUR77 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980]

Immunocytochemistry/ Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980]

Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Staining NUR77 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980]

Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980]

Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Human lymph node tissue stained with NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11). IHC-P image submitted by a verified customer review.
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Applications for NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 5 using NBP2-66980 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NGFI-B alpha/Nur77/NR4A1

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. Nak1 is a phosphoprotein and its size ranges from 67 kD to 88 kD, depending on post-translational modifications.

Long Name

Nerve Growth Factor-induced Protein I-B alpha/Nuclear Receptor Subfamily 4, Group A, Member 1

Alternate Names

HMR, NAK1, NGFIB alpha, NR4A1, Nur77, TR3

Gene Symbol

NR4A1

Additional NGFI-B alpha/Nur77/NR4A1 Products

Product Documents for NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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