Negative Control for Rabbit Monoclonal Antibody (NCRBM/1520R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53151
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # NCRBM/1520R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Negative Control for Rabbit Monoclonal protein
Localization
None
Specificity
To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54471)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Negative Control for Rabbit Monoclonal Antibody (NCRBM/1520R)
Immunohistochemistry: Negative Control for Rabbit Monoclonal Antibody (NCRBM/1520R) [NBP2-53151] -
Immunohistochemistry: Negative Control for Rabbit Monoclonal Antibody (NCRBM/1520R) [NBP2-53151] - Formalin-fixed, paraffin-embedded human oviduct stained with Cytokeratin 7 Antibody (KRT7/1499R).Applications for Negative Control for Rabbit Monoclonal Antibody (NCRBM/1520R)
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1:10-1:500
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Negative Control for Rabbit Monoclonal
Additional Negative Control for Rabbit Monoclonal Products
Product Documents for Negative Control for Rabbit Monoclonal Antibody (NCRBM/1520R)
Product Specific Notices for Negative Control for Rabbit Monoclonal Antibody (NCRBM/1520R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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