Negative Control for Mouse Monoclonal Antibody (IGG1/453)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13902
![Immunohistochemistry-Paraffin: Negative Control for Mouse Monoclonal Antibody (IGG1/453) [NBP3-13902]](https://resources.bio-techne.com/images/products/Negative-Control-for-Mouse-Monoclonal-Antibody-IGG1-453-Immunohistochemistry-Paraffin-NBP3-13902-img0001.jpg "Immunohistochemistry-Paraffin: Negative Control for Mouse Monoclonal Antibody (IGG1/453) [NBP3-13902]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # IGG1/453
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Negative Control for Mouse Monoclonal protein
Specificity
To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14085)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Negative Control for Mouse Monoclonal Antibody (IGG1/453)
Immunohistochemistry-Paraffin: Negative Control for Mouse Monoclonal Antibody (IGG1/453) [NBP3-13902]
Immunohistochemistry-Paraffin: Negative Control for Mouse Monoclonal Antibody (IGG1/453) [NBP3-13902] - Formalin-fixed, paraffin-embedded human heart stained with Negative Control for Mouse Monoclonal antibody (IGG1/453). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.Applications for Negative Control for Mouse Monoclonal Antibody (IGG1/453)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Negative Control for Mouse Monoclonal
Additional Negative Control for Mouse Monoclonal Products
Product Documents for Negative Control for Mouse Monoclonal Antibody (IGG1/453)
Product Specific Notices for Negative Control for Mouse Monoclonal Antibody (IGG1/453)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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