MyoD Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54153
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]](https://resources.bio-techne.com/images/products/MyoD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0005.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A partial recombinant protein made to an N-terminal region of the human MyoD1 protein (within residues 1-150). [Swiss-Prot# P15172]
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28569745).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MyoD Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]
Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Western Blot: MyoD AntibodyBSA Free [NBP1-54153]](https://resources.bio-techne.com/images/products/MyoD1-Antibody-Western-Blot-NBP1-54153-img0004.jpg "Western Blot: MyoD AntibodyBSA Free [NBP1-54153]")
Western Blot: MyoD AntibodyBSA Free [NBP1-54153]
Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.![Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]](https://resources.bio-techne.com/images/products/MyoD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]")
Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]
Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).Applications for MyoD Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:5000
Application Notes
This MyoD1 antibody is useful for ICC/IF and Western blot, where a band is seen ~42 kDa.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MyoD
Long Name
Myoblast Determination Protein 1
Alternate Names
bHLHc1, MYF3, MYOD1, PUM
Gene Symbol
MYOD1
UniProt
Additional MyoD Products
Product Documents for MyoD Antibody - BSA Free
Product Specific Notices for MyoD Antibody - BSA Free
Manufactured by Genomic Antibody Technology™. GAT FAQs
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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