Detects mouse Vitamin D BP in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Vitamin D BP is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Vitamin D BP Antibody

Detection of Mouse Vitamin D BP by Western Blot.

Western blot shows lysates of mouse liver. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vitamin D BP at approximately 52-58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Vitamin D BP by Simple Western^TM.

Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Vitamin D BP at approximately 58 kDa (as indicated) using 5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Vitamin D BP Antibody

Application

Recommended Usage

Simple Western

5 µg/mL
Sample:

Mouse liver tissue

Western Blot

0.5 µg/mL
Sample: Mouse liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D BP

VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively.

Long Name

Vitamin D Binding Protein

Alternate Names

DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP

Entrez Gene IDs

2638 (Human); 14473 (Mouse); 24384 (Rat)

Gene Symbol

UniProt

P21614

Additional Vitamin D BP Products

Product Documents for Mouse Vitamin D BP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Vitamin D BP Antibody

For research use only

Citations
Reviews

Mouse Vitamin D BP Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4188

Key Product Details

Species Reactivity

Validated:

Cited:

Applications