Detects mouse TNFRH3/TNFRSF26 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) TNF RI and rmTNF RII is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse TNFRH3/TNFRSF26 Antibody

Detection of Mouse TNFRH3/TNFRSF26 by Western Blot.

Western blot shows lysates of mouse thymus tissue and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5330) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TNFRH3/TNFRSF26 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of TNFRH3/TNFRSF26 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Applications for Mouse TNFRH3/TNFRSF26 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes

Western Blot

1 µg/mL
Sample: Mouse thymus tissue and mouse spleen tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNFRH3/TNFRSF26

TNFRH3 (Tumor necrosis factor receptor homolog 3; also TNFR superfamily member 26) is a 21 kDa (predicted) member of the TNF receptor superfamily. It appears to be concentrated in lymphoid cell types. Mature mouse TNFRH3 is a type I transmembrane protein that is 185 amino acid (aa) in length. It contains a 145 aa extracellular region and a 19 aa cytoplasmic domain. The extracellular region contains three TNFR-Cys repeats that characterize TNF receptors (aa 27‑143). Unlike two related TNF receptor homologs, TNFRH3 does not serve as a TRAIL decoy receptor. No ortholog to mouse TNFRH3 has been reported in human or rat.

Long Name

Tumor Necrosis Factor Receptor Homolog 3

Alternate Names

Tnfrsf24, Tnfrsf26

Entrez Gene IDs

244237 (Mouse)

Gene Symbol

TNFRSF26

UniProt

P83626

Additional TNFRH3/TNFRSF26 Products

Product Documents for Mouse TNFRH3/TNFRSF26 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse TNFRH3/TNFRSF26 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse TNFRH3/TNFRSF26 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5330

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity