Mouse Sca-1/Ly6 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1226P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 177228
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351
Leu27-Gly119
Accession # CAA28351
Specificity
Detects mouse Sca‑1/Ly6 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Sca-1/Ly6 PE-conjugated Antibody
Detection of Sca-1/Ly6 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit APC-conjugated Monoclonal Antibody (Catalog # FAB1356A) and either (A) Rat Anti-Mouse Sca-1/Ly6 PE-conjugated Monoclonal Antibody (Catalog # FAB1226P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse Sca-1/Ly6 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes gated on hematopoietic lineage negative cells
Sample: Mouse splenocytes gated on hematopoietic lineage negative cells
Reviewed Applications
Read 1 review rated 5 using FAB1226P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: Sca-1/Ly6
Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).
References
- Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
- Spangrude, G.I. et al. (1988) Science 241:58.
- Spangrude, G.I. et al. (1993) Blood 82:3327.
- Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
- Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
- Yamamoto, Y. et al. (1996, Blood 88:445.
- Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
- Welm, B.E. et al. (2002) Dev. Biol. 245:42.
- Codias, E.K. et al. (1990) J. Immunol. 144:2197.
- Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
- Codias, E.K. et al. (1990) J. Immunol. 145:1407.
- Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
Long Name
Stem Cell Antigen-1/Lymphocyte Antigen 6
Alternate Names
Ly-6, Ly6, Sca1
Entrez Gene IDs
110454 (Mouse)
Gene Symbol
LY6A
UniProt
Additional Sca-1/Ly6 Products
Product Documents for Mouse Sca-1/Ly6 PE-conjugated Antibody
Product Specific Notices for Mouse Sca-1/Ly6 PE-conjugated Antibody
For research use only
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