3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref-1/DLK1/FA1 APC-conjugated Monoclonal Antibody (Catalog # FAB8634A, filled histogram) or isotype control antibody (Catalog # IC105A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385). It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively.

Long Name

Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1

Alternate Names

DLK-1, DLK1, FA1, pG2, Pref1, ZOG

Entrez Gene IDs

8788 (Human); 13386 (Mouse)

Gene Symbol

DLK1

UniProt

Q09163

Additional Pref-1/DLK1/FA1 Products

Product Documents for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

For research use only

Citations
Reviews

Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8634A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Pref-1/DLK1/FA1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

Applications for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: Pref-1/DLK1/FA1

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Pref-1/DLK1/FA1 Products

Product Documents for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody