Detects mouse IL-27 p28/IL-30 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-27/IL-35 EBI3 subunit, recombinant human (rh) IL-27/IL-35 EBI3 subunit, or rhIL-27 heterodimer is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse IL-27 p28/IL-30 Antibody

Detection of Mouse IL‑27 p28/IL-30 by Western Blot.

Western blot shows lysates of CHO Chinese hamster ovary cell line transfected with mouse IL-27 p28/IL-30. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-27 p28/IL-30 Monoclonal Antibody (Catalog # MAB7430) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-27 p28/IL-30 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IL-27 p28/IL-30 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: CHO Chinese hamster ovary cell line transfected with mouse IL-27 p28/IL-30

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 p28/IL-30

Interleukin-27 p28, also known as IL-30, is a secreted 28 kDa protein that is considered a member of the IL-6/IL-12 interfamily cytokine family. p28/IL-30 is one of several alpha-chain proteins that can associate with various beta-chain proteins to form heterodimeric cytokines in these families. The alpha-chains (e.g. IL-6, IL-11, Cardiotrophin-1, CLC/CNTF, LIF, Oncostatin M, IL-23 p19, IL-27 p28/IL-30, IL-12/IL-35 p35) have a four-helix bundle structure, while the beta-chains (e.g. EBI-3, CLF-1, IL-12/IL-23 p40) resemble class 1 cytokine receptors. These cytokines utilize heteromeric cell surface receptors which contain shared as well as ligand-specific subunits. Divergent biological responses are obtained from the combinatorial association of cytokine subunits and their interaction with various combinations of receptor subunits. Complexity in this system is increased by the generation of soluble receptors and by the competition between proteins for shared subunit pairing (1-3). p28/IL-30 is expressed by macrophages and dendritic cells, and is upregulated in these cells by inflammatory stimuli (4-9). It was first described as a partner with EBI-3 in the heterodimeric cytokine IL-27 (4). IL-27 signals through a receptor complex composed of IL-27 R alpha/WSX-1/TCCR and gp130 (4, 10, 11). This interaction enhances the proinflammatory activation of naïve CD4⁺ T cells, NK cells, mast cells, and monocytes and the cytotoxic activity of CD8⁺ T cells (4, 10, 12). IL-27 also exhibits anti-inflammatory activity, including the induction of IL-10 production by naïve and memory T cells, the activation of regulatory T cells (Treg), and the suppression of Th17 cytokine secretion (13, 14). Alternatively, p28/IL-30 associates with CLF-1 to create a cytokine that triggers responses through IL‑27 R alpha, IL‑6 R alpha, and gp130 (6). Like IL-27, p28-CLF-1 heterodimers costimulate IFN-gamma production by NK cells, and induce IL-10 secretion by CD4⁺ T cells (6). In contrast to IL-27, however, p28-CLF-1 is reported to promote the differentiation of Th17 cells (6). A third mode of p28 action enables it to stimulate cells that express both IL‑6 R alpha and gp130, but lack IL-27 R alpha (11). Similar to the IL-6 system, the presence of IL-6 R alpha on the cell surface is not even required if p28/IL-30 associates with a soluble form of IL-6 R alpha. This combination can trigger trans signaling through gp130, a mechanism that has been demonstrated for complexes of IL-6 with soluble IL‑6 R alpha (11). Overexpression of p28/IL-30 in vivo interferes with humoral antibody responses and protects from IL-12 induced liver inflammation (7, 15). Mature mouse p28/IL-30 shares 70% and 89% amino acid sequence identity with human and rat p28, respectively.

References

Hunter, C.A. and R. Kastelein (2012) Immunity 37:960.
Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
Vignali, D.A.A. and V.K. Kuchroo (2012) Nat. Immunol. 13:722.
Pflanz, S. et al. (2002) Immunity 16:779.
Liu, J. et al. (2007) J. Exp. Med. 204:141.
Crabé, S. et al. (2009) J. Immunol. 183:7692.
Stumhofer, J.S. et al. (2010) Nat. Immunol. 11:1119.
Bosmann, M. et al. (2012) J. Immunol. 188:5086.
Dibra, D. et al. (2012) J. Immunol. 188:3709.
Pflanz, S. et al. (2004) J. Immunol. 172:2225.
Garbers, C. et al. (2013) J. Biol. Chem. 288:4346.
Schneider, R. et al. (2011) Eur. J. Immunol. 41:47.
Diveu, C. et al. (2009) J. Immunol. 182:5748.
Hall, A.O, et al. (2012) Immunity 37:511.
Dibra, D. et al. (2012) Hepatology 55:1204.

Long Name

Interleukin 27 p28

Alternate Names

IL-30, IL27 p28

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Gene Symbol

IL27

UniProt

Q8K3I6

Additional IL-27 p28/IL-30 Products

Product Documents for Mouse IL-27 p28/IL-30 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse IL-27 p28/IL-30 Antibody

For research use only

Citations
Reviews

Mouse IL-27 p28/IL-30 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7430

Key Product Details

Species Reactivity

Validated:

Cited:

Applications