Mouse ICAM-1/CD54 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF796
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑1/CD54
Gln28-Asn485
Accession # Q3U8M7
Gln28-Asn485
Accession # Q3U8M7
Specificity
Detects mouse ICAM‑1/CD54 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross‑reactivity with recombinant human ICAM-1, recombinant rat ICAM-1, and recombinant mouse ICAM-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse ICAM-1/CD54 Biotinylated Antibody
ICAM‑1/CD54 in Human Kidney.
ICAM-1/CD54 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Mouse ICAM-1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF796) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Mouse ICAM-1 / CD54 ELISA Standard Curve
Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC) was serially diluted and captured by Rat Anti-Mouse ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB796) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse ICAM-1/CD54 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
2-10 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus and immersion fixed paraffin-embedded sections of human kidney
Sample: Perfusion fixed frozen sections of mouse thymus and immersion fixed paraffin-embedded sections of human kidney
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC)
Sample: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC)
Mouse ICAM-1/CD54 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using BAF796 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.
References
- Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
- Siu, G. et al. (1989) J. Immunol. 143:3813.
- Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
UniProt
Additional ICAM-1/CD54 Products
Product Documents for Mouse ICAM-1/CD54 Biotinylated Antibody
Product Specific Notices for Mouse ICAM-1/CD54 Biotinylated Antibody
For research use only
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