Mouse GFR alpha-3/GDNF R alpha-3 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2645S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse GFRα-3/GDNF Rα-3
Glu34-Arg379
Accession # AAB70931
Glu34-Arg379
Accession # AAB70931
Specificity
Detects mouse GFR alpha-3/GDNF R alpha-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GFR alpha-3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: GFR alpha-3/GDNF R alpha-3
Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha-3) is a member of the GDNF family of receptors. It is one of four known members, all of which contain three conserved cysteine repeats (1, 3, 4). It is synthesized as a 397 aa precursor, with a hydrophobic signal sequence of 28 aa, a 343 aa mature segment, and a propeptide segment of 26 aa that is removed in the mature protein. The mature globular membrane glycoprotein has an approximate molecular weight of 50 kDa (5), contains three potential N-linked glycosylation sites, and a hydrophobic stretch of residues at its COOH terminus that comprises a GPI linkage sequence (2, 3, 5). Mouse GFR alpha-3 shares 81% aa sequence identity with human GFR alpha-3. High-level expression of GFR alpha-3 is observed only during early stages of neurogenesis in the central nervous system and in developing and adult peripheral nerves, organs, and ganglia (2, 3, 5, 7). The expression and proportions of GFR alpha-3 are closely linked to those of the Ret receptor tyrosine kinase, particularly in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (3). GFR alpha-3 associates with Ret to form the receptor complex for the GDNF family ligand artemin, which, in turn, activates the complex (1, 6, 7). The activated complex’s signal is required for the development and maintenance of superior cervical ganglion (SCG), specifically the rostral migration of SCG precursors between embryonic days 11.5 and 14.5, and the survival of SCG neurons after birth (8).
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 3
Alternate Names
GDNF R alpha-3, GFR alpha3, GFRa-3, GFRA3
Gene Symbol
GFRA3
UniProt
Additional GFR alpha-3/GDNF R alpha-3 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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