Mouse Cystatin D/Cst10 Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6255X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin D/Cst10
Asn30-Phe148
Accession # NP_067380
Asn30-Phe148
Accession # NP_067380
Specificity
Detects mouse Cystatin D/Cst10 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) Cst1, rhCst2, and rhCst3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Cystatin D/Cst10
Cystatin D (Cystatin 10; also Carminerin) is a 17 kDa member of the type 2 cystatin family of proteins. It is expressed in salivary gland, skin, prehypertrophic and hypertrophic chondrocytes. Within the context of endochondral ossification, it may both induce chondrocyte apoptosis and initiate the process of cartilage calcification. Although Cystatin D contains a key cystatin motif (Gln-x-Val-x-Gly), Cystatin D does not appear to inhibit cysteine proteases. Mouse Cystatin D is 148 amino acids (aa) in length. It contains one cystatin-like domain (aa 39-145). There are two potential splice variants. One shows a deletion of aa 84-121, while a second shows a Val substitution for aa 122-148. Full-length mouse Cystatin D shares 78% aa identity with rat Cystatin D. There appears to be no direct human counterpart to mouse Cystatin D, as both human cystatin-C and -D share less that 40% aa identity with mouse Cystatin D.
Alternate Names
Cst10, Cystatin 10
Gene Symbol
CST10
UniProt
Additional Cystatin D/Cst10 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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