Detects mouse CRELD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CRELD2 is observed and less than 1% cross-reactivity with recombinant mouse CRELD1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CRELD2 Antibody

Detection of CRELD2 by Western Blot

Lack of CRELD2 effects in murine embryonic fibroblasts.(a) EdU incorporation in mouse embryonic fibroblasts (MEFs) cultured for 24 hours in the absence or presence of transforming growth factor beta 1 (TGF beta1, 2 ng/mL) or CRELD2 (100 ng/mL). 3–6 experiments. (b) Recovery 16 hours after scratch injury of MEF monolayers in the absence or presence of TGF beta1 or CRELD2. 4 experiments. (c) Smooth muscle actin alpha 2 (Acta2) and (d) collagen type I alpha1 (Col1a1) mRNA expression (RT-qPCR, normalized to glyceraldehyde-3-phosphate dehydrogenase [Gapdh]) in MEFs cultured for 24 hours in the absence or presence of TGF beta1 or CRELD2. 6 experiments. (a–d) *P < 0.05, **P < 0.01, ***P < 0.001 (1-way ANOVA with Tukey test). Individual data points, mean values, and SEMs are shown. (e) Exemplary immunoblots (out of 3) showing phosphorylated (P)-SMAD2 (S465/S467), P-SMAD3 (S423/S425), and SMAD2 and SMAD3 expression in MEFs stimulated with TGF beta1 in the absence or presence of CRELD2 (added 30 min prior to TGF beta1).Source data Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39196188), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CRELD2 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CRELD2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRELD2

CRELD2 is a ubiquitously expressed protein that is the second member of the CRELD family of matricellular proteins. Mouse CRELD2 is synthesized as a 350 amino acid precursor protein with a signal peptide, a highly conserved domain rich in glutamic acid and tryptophan (WE), and EGF-like repeats. Human CRELD2 has been shown to interact with the alpha4 subunit of the nicotinic acetylcholine receptor and function as a specific regulator of receptor expression. Mouse CRELD2 shares 77% amino acid sequence homology with the human CRELD2 alpha isoform.

Long Name

Cysteine-rich Protein With EGF-like Domains 2

Alternate Names

cysteine-rich with EGF-like domain protein 2, cysteine-rich with EGF-like domains 2, DKFZp667O055, MGC11256

Entrez Gene IDs

79174 (Human); 76737 (Mouse)

Gene Symbol

CRELD2

UniProt

Q9CYA0 (Mouse)

Additional CRELD2 Products

Product Documents for Mouse CRELD2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CRELD2 Antibody

For research use only

Citations
Reviews

Mouse CRELD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3686

Key Product Details

Species Reactivity

Validated:

Cited:

Applications