Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Complement Factors B, D,H, I or rhGranzyme A is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse Complement Factor D/Adipsin Antibody

Detection of Mouse Complement Factor D/Adipsin by Western Blot.

Western blot shows lysates of mouse adipose tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor D/Adipsin Monoclonal Antibody (Catalog # MAB5430) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Complement Factor D/Adipsin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Complement Factor D/Adipsin Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse Complement Factor D, see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Mouse adipose tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Complement Factor D contains one peptidase S1 domain that cleaves an Arg‑Lys bond. There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse Complement Factor D shares 85% and 68% aa identity with rat and human Complement Factor D, respectively.

Alternate Names

Adipsin, ADN, AMBP-1, CFD, PFD

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Gene Symbol

CFD

UniProt

P03953

Additional Complement Factor D/Adipsin Products

Product Documents for Mouse Complement Factor D/Adipsin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Complement Factor D/Adipsin Antibody

For research use only

Citations
Reviews

Mouse Complement Factor D/Adipsin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5430

Key Product Details

Species Reactivity

Validated:

Cited:

Applications