Applications for Mouse Collagen I alpha 1 Antibody
Recommended Concentration
Sample
Western Blot
1 µg/mL
MEF mouse embryonic feeder cells
Simple Western
10 µg/mL
MEF mouse embryonic feeder cells
Immunohistochemistry
3-25 µg/mL
Perfusion fixed paraffin-embedded sections of mouse kidney and pancreas
Immunocytochemistry
3-25 µg/mL
Immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line
Multiplex Immunofluorescence
25 µg/mL
Immersion fixed paraffin-embedded sections of mouse colon
Scientific Data Examples for Mouse Collagen I alpha 1 Antibody
Multiplex Immunofluorescence
COL1A1 in Mouse Colon via seqIF™ staining on COMET™
COL1A1 was detected in immersion fixed paraffin-embedded sections of mouse Colon using Rat Anti-Mouse COL1A1, Monoclonal Antibody (Catalog #MAB11700) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to connective tissue. Protocol available in COMET™ Panel Builder.
Immunohistochemistry
Detection of Collagen I alpha 1 in Mouse Kidney.
Collagen I alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Detection of Collagen I alpha 1 in Mouse Pancreas.
Collagen I alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse pancreas using Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
Detection of Collagen I alpha 1 in 3T3-L1 cells.
Collagen I alpha 1 was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast adipose-like cell line (Positive) and absent in RAW 264.7 mouse monocyte/macrophage cell line (Negative) using Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot
Detection of Mouse Collagen I alpha 1 by Western Blot.
Western Blot shows lysates of MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Collagen I alpha 1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Simple Western
Detection of Mouse Collagen I alpha 1 by Simple WesternTM.
Simple Western lane view shows lysates of MEF mouse embryonic feeder cells, loaded at 0.1 mg/ml. A specific band was detected for Collagen I alpha 1 at approximately 226 kDa (as indicated) using 10 µg/ml of Rat Anti-Mouse Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator
Background: Collagen I alpha 1
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides (1). The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues (1). Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described (2). This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
Item
Value
References
Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
Han, S. et al. (2008) J. Mol. Biol. 383:122.
Entrez Gene IDs
1277 (Human); 12842 (Mouse); 29393 (Rat)
Alternate Names
Alpha-1 type I collagen, COL-IA1, COL1A1, Collagen 1, Collagen 1 alpha 1, Collagen I alpha 1, Collagen Type I Alpha 1 Chain, Collagen-1, Collagen1, EDSARTH1, OI4, Type I Procollagen Alpha 1 Chain, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, pro-alpha-1 collagen type 1
Bio-Techne uses cookies to provide you with a great website experience. By continuing to use this website you acknowledge this and agree to our cookie policy. Learn more.
