In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse CD97 v2 APC-conjugated Antibody

Detection of CD97 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD97 v2 APC-conjugated Monoclonal Antibody (Catalog # FAB3734A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD97 v2 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD97

CD97 is an approximately 100 kDa N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. It contains a 510 amino acid (aa) N-terminal extracellular region with four EGF-like domains and a juxtamembrane GPS motif which enables proteolytic shedding of the N-terminal extracellular domain. The alternately spliced variant 2 of CD97 exhibits a 94 aa deletion that results in loss of the third EGF-like repeat. CD97 is expressed on monocytes, macrophages, T cells, B cell subsets, dendritic cells, hematopoietic progenitor cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, and Integrin although interaction with these molecules can vary between splice forms of CD97. CD97 participates in neutrophil migration, host defense, and angiogenesis. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.

Alternate Names

CD97

Entrez Gene IDs

976 (Human); 26364 (Mouse)

Gene Symbol

ADGRE5

UniProt

AAH06676

Additional CD97 Products

Product Documents for Mouse CD97 v2 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD97 v2 APC-conjugated Antibody

For research use only

Citations
Reviews

Mouse CD97 v2 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3734A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD97 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD97 v2 APC-conjugated Antibody

Detection of CD97 in Mouse Splenocytes by Flow Cytometry.

Applications for Mouse CD97 v2 APC-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: CD97

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CD97 Products

Product Documents for Mouse CD97 v2 APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Mouse CD97 v2 APC-conjugated Antibody