CD47, also known as Integrin‑Associated Protein (IAP) and OA3, is a 40‑60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Mouse CD47 is an integral membrane protein that consists of a 122 amino acid (aa) extracellular domain (ECD) with a single Ig‑like domain, five membrane-spanning regions with short intervening loops, and a 16 aa C‑terminal cytoplasmic tail (3). Alternate splicing of mouse CD47 generates an additional isoform with an insertion of 21 aa following the Ig‑like domain (3). Within the N‑terminal ECD, mouse CD47 shares 63% and 84% aa sequence identity with human and rat CD47, respectively. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2‑mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage‑mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5‑8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection (16). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9‑11). CD47 also associates in cis with Integrins alpha4 beta1, alphaV beta3, alpha2b beta3, and alpha2 beta1 which can positively or negatively modulate Integrin-mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin‑1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP‑1 induces giant cell formation and osteoclast differentiation (15).

References

Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
Lindberg, F.P. et al. (1993) J. Cell Biol. 123:485.
Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
Oldenborg, P.-A. et al. (2000) Science 288:2051.
Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
Stefanidakis, M. et al. (2008) Blood 112:1280.
de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
Kukreja, A. et al. (2009) Blood 114:3413.
Wang H. et al. (2007) Blood 109:836.

Alternate Names

CD47, IAP, MER6, OA3

Entrez Gene IDs

961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)

Gene Symbol

CD47

UniProt

NP_034711

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Product Specific Notices for Mouse CD47 Alexa Fluor™ Plus 647-conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Mouse CD47 Alexa Fluor™ Plus 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB18661AFP647

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Applications for Mouse CD47 Alexa Fluor™ Plus 647-conjugated Antibody

Blockade of Receptor-ligand Interaction

CyTOF-ready

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Background: CD47