Mouse CCR7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3477
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Neutralization, Flow Cytometry, Immunocytochemistry, CyTOF-reported
Cited:
Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development, In vivo assay
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 4B12
Product Specifications
Immunogen
RBC-2H3 cells expressing mouse CCR7
Accession # NP_031745
Accession # NP_031745
Specificity
Detects mouse CCR7.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCR7 Antibody
CCR7 in Mouse Splenocytes.
CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Chemotaxis Induced by CCL19/MIP-3 beta and Neutralization by Mouse CCR7 Antibody.
Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND50 is typically 2-10 µg/mL.
Detection of CCR7 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CCR7 Antibody
Application
Recommended Usage
CyTOF-reported
Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse CD3+ splenocytes
Sample: Mouse CD3+ splenocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed non-stimulated mouse splenocytes
Sample: Immersion fixed non-stimulated mouse splenocytes
Neutralization
Measured by its ability to neutralize CCL19/MIP-3 beta-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR7. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL19/MIP-3 beta.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR7
Alternate Names
BLR2, CC-CKR-7, CCR7, CD197, CDw197, CMKBR7, EBI1
Gene Symbol
CCR7
UniProt
Additional CCR7 Products
Product Documents for Mouse CCR7 Antibody
Product Specific Notices for Mouse CCR7 Antibody
For research use only
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