Mouse beta-Actin Antibody

Name: Mouse beta-Actin Antibody
Brand: R&D Systems
SKU: MAB11702

R&D Systems, part of Bio-Techne | Catalog # MAB11702

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 1104805

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Product Specifications

Immunogen

Synthetic Peptide
Accession # P60710

Specificity

Detects a synthetic peptide specific for mouse beta-actin around amino acid 50 in Direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse beta-Actin Antibody

beta-Actin Antibody in Mouse Stomach viaseqIF™ staining on COMET™

beta-Actin was detected in perfusion fixed paraffin-embedded sections of mouse Stomach using Rat Anti-Mouse beta-Actin, Monoclonal Antibody (Catalog #MAB11702) at 0.25ug/mL at 37° Celsius for 2 minutes.Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Rat IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

beta-Actin Antibody in Mouse colon via seqIF™ staining on COMET™

beta-Actin was detected in immersion fixed paraffin-embedded sections of mouse colon using Rat Anti-Mouse beta-Actin, Monoclonal Antibody (Catalog # MAB11702) at 0.25ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Rat IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

beta-Actin Antibody in Mouse liver via seqIF™ staining on COMET™

beta-Actin was detected in immersion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse beta-Actin, Monoclonal Antibody (Catalog # MAB11702) at 0.25ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Rat IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of beta-Actin in Mouse Heart.

beta-Actin was detected in perfusion fixed paraffin-embedded sections of mouse heart using Rat Anti-Mouse beta-Actin Monoclonal Antibody (Catalog # MAB11702) at 0.05 µg/ml overnight at 4 °C. Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of beta-Actin in Mouse Liver.

beta-Actin was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse beta-Actin Monoclonal Antibody (Catalog # MAB11702) at 0.05 µg/ml overnight at 4 °C. Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of beta-Actin in Mouse Lung.

beta-Actin was detected in perfusion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse beta-Actin Monoclonal Antibody (Catalog # MAB11702) at 0.05 µg/ml overnight at 4 °C. Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of beta-Actin in 3T3-L1 Mouse Cell Line.

beta-Actin was detected in immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line using Rat Anti-Mouse beta-Actin Monoclonal Antibody (Catalog # MAB11702) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human, Mouse and Rat beta-Actin by Western Blot.

Western Blot shows lysates of human cerebellum, mouse cerebellum, rat cerebellum, C2C12 mouse myoblast cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/ml of Rat Anti-Mouse beta-Actin Monoclonal Antibody (Catalog # MAB11702) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for beta-Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse beta-Actin by Simple Western^TM.

Left: Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.1 mg/ml. A specific band was detected for beta-Actin at approximately 49 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Rat Anti-Mouse beta-Actin Monoclonal Antibody (Catalog # MAB11702) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Rat Anti-Mouse beta-Actin Monoclonal Antibody (Catalog # MAB11702) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the C2C12 mouse myoblast cell line.

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Applications for Mouse beta-Actin Antibody

Application

Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line.

Immunohistochemistry

0.05-10 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of Mouse Heart, Liver and Lung.

Multiplex Immunofluorescence

0.25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse stomach, colon and liver

Simple Western

10-50 µg/mL
Sample: C2C12 mouse myoblast cell line

Western Blot

0.5 µg/mL
Sample: Human cerebellum, mouse cerebellum, rat cerebellum, C2C12 mouse myoblast cell line and NIH-3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Actin

Beta actin (ACTB) is a canonical member of the actin family of proteins, with a molecular weight of approximately 42 kDa. Actins are highly conserved proteins that play pivotal roles in various cellular processes, including the maintenance of the cytoskeleton and cell motility, division, and signaling. ACTB is ubiquitously expressed in all eukaryotic cells and is crucial for structural integrity and motility. It is involved in essential biological processes such as cellular trafficking and the maintenance of cell shape and polarity. Dysregulation of ACTB expression or function can lead to notable pathologies, including cancer progression, where it is implicated in tumor cell migration, invasion, and metastasis. Mutations in the ACTB gene are also associated with developmental disorders and conditions like Baraitser-Winter syndrome. Additionally, ACTB plays a critical role in intracellular transport processes and the functioning of the actin cytoskeleton, highlighting its potential as a biomarker for various diseases and a target for therapeutic interventions.

References

Pollard, T. D., & Cooper, J. A. (2009). Actin, a central player in cell shape and movement. Science, 326(5957), 1208-1212. doi: 10.1126/science.1175862.
Perrin, B. J., & Ervasti, J. M. (2010). The actin gene family: Function follows isoform. Cytoskeleton, 67(10), 630-634. doi: 10.1002/cm.20475.
Jones, S. L., Korobova, F., Svitkina, T. (2019). Origin of the actin cytoskeleton. Nature Reviews Molecular Cell Biology, 20(11), 675-689. doi: 10.1038/s41580-019-0164-5.

Alternate Names

ACTB, betaActin

Entrez Gene IDs

60 (Human); 11461 (Mouse); 81822 (Rat)

Gene Symbol

ACTB

UniProt

P60710 (Mouse)

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Product Specific Notices for Mouse beta-Actin Antibody

For research use only

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