Detects mouse APLP-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human APLP-1 is observed and less than 1% cross-reactivity with recombinant mouse APLP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse APLP-1 Antibody

Detection of Human and Mouse APLP-1 by Simple Western^TM.

Simple Western lane view shows lysates of human brain (hippocampus) and mouse brain (hippocampus), loaded at 0.2 mg/mL. A specific band was detected for APLP-1 at approximately 119 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse APLP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3179) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse APLP-1 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse APLP-1, see our available Western blot detection antibodies

Simple Western

25 µg/mL
Sample: Human brain (hippocampus) and mouse brain (hippocampus)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse APLP-1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APLP-1

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and is 89% identical between human and mouse.

Long Name

Amyloid-like Protein 1

Alternate Names

APLP1

Entrez Gene IDs

333 (Human); 11803 (Mouse); 502317 (Rat)

Gene Symbol

APLP1

UniProt

Q03157

Additional APLP-1 Products

Product Documents for Mouse APLP-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse APLP-1 Antibody

For research use only

Citations
Reviews

Mouse APLP-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3179

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all APLP-1 Antibodies »

Product Specifications

Immunogen

Specificity