MLH1 Antibody (S07-5D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14898

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14898-100ul
NBP3-14898-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-5D3 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MLH-1 Antibodies »

Product Specifications

Immunogen

Recombinant protein of human MLH1 (Uniprot#: P40692)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MLH1 Antibody (S07-5D3) (NBP3-14898) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MLH1 Antibody (S07-5D3)

Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898]

Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898]

Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898] - Western blot detection of MLH1 in Hela, A549, HL-60, U2OS cell lysates using MLH1 Rabbit mAb (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.
MLH1 Antibody (S07-5D3)

Immunocytochemistry/Immunofluorescence: MLH1 Antibody (S07-5D3) [NBP3-14898] -

Immunocytochemistry/Immunofluorescence: MLH1 Antibody (S07-5D3) [NBP3-14898] - Immunocytochemistry of MLH1 (green) in hela using NBP3-14898 at dilution 1/50, and DAPI (blue)

Applications for MLH1 Antibody (S07-5D3)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLH-1

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPCC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Long Name

mutL Homolog 1

Alternate Names

MLH1

Gene Symbol

MLH1

Additional MLH-1 Products

Product Documents for MLH1 Antibody (S07-5D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MLH1 Antibody (S07-5D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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