MHC Class II Antibody (OX-6) - BSA Free

Name: MHC Class II Antibody (OX-6) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-43538

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43538

Submit a review

Product Datasheet / COA

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Radioimmunoassay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ Clone # OX-6

Format

BSA Free

Concentration

1 mg/ml

View all MHC Class II Antibodies »

Product Specifications

Immunogen

This antibody was prepared by subcutaneously injecting non-polymorphic MHC class II (Ia-like glycoproteins) from the membranes of rat thymocytes into BALB/c mice.

Specificity

This antibody recognises a monomorphic determinant of the rat MHC Class II (I-A) antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. It also cross-reacts with mouse MHC, specifically I-A[k] and I-A[s]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Novus Biologicals Mouse MHC Class II Antibody (OX-6) - BSA Free (NBP3-43482) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MHC Class II Antibody (OX-6) - BSA Free

Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] -

Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with NBP3-43538 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43538, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] -

Western Blot: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] - Rat spleen (A), rat thymus (B) and rat lymph node (C) lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-43538 at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for MHC Class II is ~24.5-31.6kDa, depending on the isoform and post-translational modification. NBP3-43538 successfully detected MHC Class II in rat spleen, thymus and lymph node lysates.

Flow Cytometry: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] -

Flow Cytometry: MHC Class II Antibody (OX-6) - BSA Free [NBP3-43538] - Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or NBP3-43538 at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.