MHC Class II Antibody (M5/114) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21789
![Flow Cytometry: MHC Class II Antibody (M5/114) - Azide and BSA Free [NBP2-21789]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-M5-114-Azide-Free-Flow-Cytometry-NBP2-21789-img0001.jpg "Flow Cytometry: MHC Class II Antibody (M5/114) - Azide and BSA Free [NBP2-21789]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation, Functional, CyTOF-ready
Cited:
Immunohistochemistry, Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # M5/114
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Activated C57BL/6 mouse spleen cells
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34166814).
Specificity
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for MHC Class II Antibody (M5/114) - Azide and BSA Free
Flow Cytometry: MHC Class II Antibody (M5/114) - Azide and BSA Free [NBP2-21789]
Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.Applications for MHC Class II Antibody (M5/114) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
2 ug/ml
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Frozen
1:50-1:300
Immunohistochemistry-Paraffin
1:50-1:300
Immunoprecipitation
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
Functional Application: Blocking of T cell proliferative responses. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MHC Class II
Alternate Names
class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbol
HLA-DMA
Additional MHC Class II Products
Product Documents for MHC Class II Antibody (M5/114) - Azide and BSA Free
Product Specific Notices for MHC Class II Antibody (M5/114) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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