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metallothionein Antibody (UC1MT) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97493

Novus Biologicals, part of Bio-Techne
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NBP1-97493-0.025mg
NBP1-97493-0.05mg
NBP1-97493-0.2mg

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Equine, Fish, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # UC1MT

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Cross-linked rabbit liver Metallothionein I and II.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for metallothionein Antibody (UC1MT) - BSA Free

Western Blot: Mouse Monoclonal metallothionein Antibody (UC1MT) [NBP1-97493] -

Lane 1: MW marker, Lane 2: MT I, Lane 3: MT II, Lane 4: Mummichog CdCl2.

Immunohistochemistry-Paraffin: Mouse Monoclonal metallothionein Antibody (UC1MT) [NBP1-97493] -

Human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10ug/ml.

Applications for metallothionein Antibody (UC1MT) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
Immunohistochemistry-Paraffin and detects a band of ~15kDa by Western blot.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: metallothionein

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Additional metallothionein Products

Product Documents for metallothionein Antibody (UC1MT) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for metallothionein Antibody (UC1MT) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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