Key Product Details

Species Reactivity

Bacteria

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F5

Format

BSA Free

Concentration

1 mg/ml

View all metallothionein Antibodies »

Product Specifications

Immunogen

Recombinant full length Pseudomonas aeruginosa Metallothionein protein

Reactivity Notes

Bacteria (Pseudomonas aeruginosa)

Specificity

Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for metallothionein Antibody (1F5) - BSA Free

Western Blot: metallothionein Antibody (1F5) [NBP3-18261]

Western Blot: metallothionein Antibody (1F5) [NBP3-18261]

Western Blot: metallothionein Antibody (1F5) [NBP3-18261] - Western Blot analysis of Pseudomonas aeruginosa metallothionein (PmtA) GST tagged showing detection of 36 kDa metallothionein protein using Mouse Anti-metallothionein Monoclonal Antibody, Clone 1F5 (NBP3-18261). Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa metallothionein (PmtA) GST tagged. Load: 1 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-metallothionein Monoclonal Antibody (NBP3-18261) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.

Applications for metallothionein Antibody (1F5) - BSA Free

Application
Recommended Usage

ELISA

1:800

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS pH 7.4, 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: metallothionein

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Additional metallothionein Products

Product Documents for metallothionein Antibody (1F5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for metallothionein Antibody (1F5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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