Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34797
![Western Blot: Melan-A/MART-1 Antibody (SPM555)Azide and BSA Free [NBP2-34797]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-SPM555-Azide-and-BSA-Free-Western-Blot-NBP2-34797-img0003.jpg "Western Blot: Melan-A/MART-1 Antibody (SPM555)Azide and BSA Free [NBP2-34797]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM555
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)
Localization
Cytoplasmic
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide.
Antibody without azide - store at -20 to -80C.
Antibody without azide - store at -20 to -80C.
Scientific Data Images for Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free
Western Blot: Melan-A/MART-1 Antibody (SPM555)Azide and BSA Free [NBP2-34797]
Western Blot: Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free [NBP2-34797] - Western Blot Analysis of COLO 38 cell lysate using Melan-A/MART-1 MAb (SPM555).![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free [NBP2-34797]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-SPM555-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34797-img0002.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free [NBP2-34797]")
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free [NBP2-34797]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free [NBP2-34797] - Formalin-paraffin human melanoma stained with Melan-A MAb (SPM555). Note cytoplasmic staining of cells.Applications for Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Melan-A/MART-1
Alternate Names
Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA
Gene Symbol
MLANA
Additional Melan-A/MART-1 Products
Product Documents for Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free
Product Specific Notices for Melan-A/MART-1 Antibody (SPM555) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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