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Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24314

Recombinant Monoclonal Antibody
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NBP3-24314
Conjugate
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # rMLANA/9404

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human Melan-A/MART-1 protein

Localization

Cytoplasm.

Specificity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Prepared in 10mM PBS WITHOUT BSA & azide.

Antibody without azide - store at -20 to -80C

Scientific Data Images for Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free

Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free [NBP3-24314] -

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free [NBP3-24314] -

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free [NBP3-24314] - Formalin-fixed, paraffin-embedded human skin stained with Melan-A/MART-1 Antibody (rMLANA/9404). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Melan-A/MART-1 Antibody (rMLANA/9404) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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