MDM2/HDM2 Antibody (SMP14) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2736
![Western Blot: MDM2/HDM2 Antibody (SMP14)BSA Free [NB100-2736]](https://resources.bio-techne.com/images/products/MDM2-HDM2-Antibody-SMP14-Western-Blot-NB100-2736-img0001.jpg "Western Blot: MDM2/HDM2 Antibody (SMP14)BSA Free [NB100-2736]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SMP14
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]
Epitope
Amino acids 154 - 167.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 15625077). Mouse reactivity reported in scientific literature (PMID: 25981963).
Localization
Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).
Specificity
The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MDM2/HDM2 Antibody (SMP14) - BSA Free
Western Blot: MDM2/HDM2 Antibody (SMP14)BSA Free [NB100-2736]
Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]](https://resources.bio-techne.com/images/products/MDM2-HDM2-Antibody-SMP14-Immunohistochemistry-Paraffin-NB100-2736-img0003.jpg "Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]")
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]](https://resources.bio-techne.com/images/products/MDM2-HDM2-Antibody-SMP14-Immunohistochemistry-Paraffin-NB100-2736-img0002.jpg "Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]")
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) - BSA Free [NB100-2736]
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.Applications for MDM2/HDM2 Antibody (SMP14) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Frozen
1:200-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
This antibody has also been reported useful in IHC on resin tissue sections. Staining of IHC-P tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Reviewed Applications
Read 1 review rated 4 using NB100-2736 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDM2/HDM2
Long Name
Double Minute 2 Protein
Alternate Names
HDM2
Entrez Gene IDs
4193 (Human)
Gene Symbol
MDM2
UniProt
Additional MDM2/HDM2 Products
Product Documents for MDM2/HDM2 Antibody (SMP14) - BSA Free
Product Specific Notices for MDM2/HDM2 Antibody (SMP14) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...