MCM7 Antibody (15E1-R)

Western Blot: MCM7 Antibody (15E1-R) [NBP3-32580]

Western Blot: MCM7 Antibody (15E1-R) [NBP3-32580] - Western blot analysis of MCM7 on different lysates with Mouse anti-MCM7 antibody (NBP3-32580) at 1/1,000 dilution.

Lane 1: HL-60 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: SW480 cell lysate
Lane 6: LNCaP cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: COS-1 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 81 kDa
Observed band size: 81 kDa

Exposure time: 1 minute 55 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.