Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 7/4

Format

BSA Free

Concentration

1.0 mg/ml

View all Ly-6B.2 Antibodies »

Product Specifications

Immunogen

Cultured bone marrow cells

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).

Specificity

Recognizes Ly6B.2, a polymorphic 40 kDa antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4:129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains Negative/Weak for 7/4:A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

Fusion Partners Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.

Scientific Data Images for Ly-6B.2 Antibody (7/4) - BSA Free

Immunocytochemistry/ Immunofluorescence: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]

Immunocytochemistry/ Immunofluorescence: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]

Immunocytochemistry/Immunofluorescence: Ly-6B.2 Antibody (7/4) [NBP2-13077] - IF staining of mouse Peyer's patch with Ly-6B.2 Antibody (7/4), NBP2-13077, green; counterstained for macrophages (F4/80), red and nuclei, blue.
Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]

Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]

Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) [NBP2-13077] - Ly-6B.2 immunoreactivity in mouse bone marrow. Primary antibody was used at 125 ng/mL and was incubated with tissue sections for 30 minutes at room temperature. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]

Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]

Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) [NBP2-13077] - Staining of C57BL6 mouse spleen cryosection with Ly-6B.2 antibody (7/4).

Applications for Ly-6B.2 Antibody (7/4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-13077 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ly-6B.2

Additional Ly-6B.2 Products

Product Documents for Ly-6B.2 Antibody (7/4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ly-6B.2 Antibody (7/4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.