Luciferase Antibody [DyLight 405]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1677V
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Firefly
Applications
Western Blot, ELISA
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Luciferase [Photinus pyralis (Firefly)]
Reactivity Notes
No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Specificity
No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Luciferase Antibody [DyLight 405]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody has been tested in Western Blot. Expect a band ~60kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunofluorescence, immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Luciferase
The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.
References
1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9
2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005
3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b
4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036
Alternate Names
LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase
Additional Luciferase Products
Product Documents for Luciferase Antibody [DyLight 405]
Product Specific Notices for Luciferase Antibody [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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