Lipid A LPS Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Lipid A LPS Antibody - BSA Free [NB100-64484]

Immunocytochemistry/Immunofluorescence: Lipid A LPS Antibody [NB100-64484] - IF assay using NB100-64484 at a 1:100 dilution to stain Shigella Sonnei using above protocol. The magnification is 400X.

Western Blot: Lipid A LPS Antibody - BSA Free [NB100-64484] -

Molecular painting. GPI-anchored GFP only (GFP protein), OMVs only (OMV) and a mix of both (OMV + GFP) were incubated and OMV were separated from not associated material by ultracentrifugation. (A) Fluorometric data after molecular painting. Significantly higher fluorescence was observed in the OMV + GFP samples than in control samples containing GFP protein alone (GFP protein) and OMVs alone (OMV). Residual fluorescence is most likely due to residual GFP protein and vesicle autofluorescence. The p-values derived from t-test are indicated. (B) Immunoblotting for GFP and LPS. While a signal for GFP can only be found in the OMV + GFP samples, LPS is detected in all samples containing OMVs. (C) Flow cytometry analysis. Porcine PBMCs were incubated with OMVs to test the immune stimulatory potential of the vesicles. The B-cell proliferation marker Ki67 was used to show activation. The chemical compound R848 serves as an unspecific stimulator. In the absence of OMVs or in the presence of GPI-anchored proteins only, no significant stimulation is observed (no OMV and GPI-AP samples). OMVs show prominent B-cell stimulation, independent of whether molecular painting has been performed (compare samples OMV un and OMV tr). All error bars indicate standard deviations. N ≥ 2. The p-values are derived from t-test analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34199851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.