LAP (TGF-beta 1) Antibody (27232) [Allophycocyanin/Cy7]
Novus Biologicals, part of Bio-Techne | Catalog # FAB2463APCCY7
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 27232
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Specificity
Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta1, rhTGF‑ beta2, rhTGF‑ beta1.2, rhTGF‑ beta3, or rhTGF‑ alpha.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for LAP (TGF-beta 1) Antibody (27232) [Allophycocyanin/Cy7]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: LAP (TGF-beta 1)
TGF-beta 1 is expressed as a proprotein that is cleaved within the trans-Golgi to yield a latency-associated peptide (LAP) and the mature TGF-beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain noncovalently associated after secretion, forming the small latent TGF-beta 1 complex. Purified LAP can also associate with active TGF-beta and neutralize TGF-beta activity. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that can interact with the extracellular matrix. TGF-beta activation from latency is controlled by multiple factors including Plasmin, MMP-9, Thrombospondin-1, and various Integrins. The TGF-beta 1 LAP is capable of complexing with and inactivating all other human TGF-beta isoforms and those of most other species.
Long Name
Latency-associated Peptide
Alternate Names
LAP (TGFbeta 1)
Gene Symbol
TGFB1
Additional LAP (TGF-beta 1) Products
Product Documents for LAP (TGF-beta 1) Antibody (27232) [Allophycocyanin/Cy7]
Product Specific Notices for LAP (TGF-beta 1) Antibody (27232) [Allophycocyanin/Cy7]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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