L-Asparaginase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78086

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

E. coli

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

LYOPH mg/ml

View all L-Asparaginase Antibodies »

Product Specifications

Immunogen

L-L-Asparaginase [E. coli] (Uniprot: P00805)

Reactivity Notes

L-Asparaginase. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Asparaginase [E.coli]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for L-Asparaginase Antibody - BSA Free

Western Blot: L-Asparaginase Antibody [NBP1-78086]

Western Blot: L-Asparaginase Antibody [NBP1-78086]

Western Blot: L-Asparaginase Antibody [NBP1-78086] - Lane 1: L-Asparaginase. Lane 2: none. Load: 100 ug per lane. Primary antibody: L-Asparaginase antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 32 kDa for L-Asparaginase. Other band(s): L-Asparaginase splice variants and isoforms.

Applications for L-Asparaginase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:25000

Immunoprecipitation

1:100

Western Blot

1:500-1:3000
Application Notes
This product has been tested by ELISA and western blot and is assayed in western blot and against 1.0 ug of Asparaginase [E. coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:400,000 is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: L-Asparaginase

Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.

Alternate Names

AnsA, AnsB, Asparaginase, Colaspase, Cytoplasmic asparaginase I, L ASNase I, L ASNase II, L asparaginase 1, L asparaginase 2, L asparaginase I, L asparaginase II, L asparaginase II precursor, L asparagine amidohydrolase I, L asparagine amidohydrolase II

Entrez Gene IDs

1039460 (E. coli)

UniProt

P00805 (E. coli)

Additional L-Asparaginase Products

Product Documents for L-Asparaginase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for L-Asparaginase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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