IZUMO1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83086
![Western Blot: IZUMO1 Antibody [NBP1-83086]](https://resources.bio-techne.com/images/products/IZUMO1-Antibody-Western-Blot-NBP1-83086-img0011.jpg "Western Blot: IZUMO1 Antibody [NBP1-83086]")
![Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]](https://resources.bio-techne.com/images/products/IZUMO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83086-img0012.jpg "Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Porcine
Cited:
Human, Porcine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry
Cited:
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQM
Reactivity Notes
Porcine reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit IZUMO1 Antibody - BSA Free (NBP1-83086) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-IZUMO1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for IZUMO1 Antibody - BSA Free
![Western Blot: IZUMO1 Antibody [NBP1-83086]](https://resources.bio-techne.com/images/products/IZUMO1-Antibody-Western-Blot-NBP1-83086-img0017.jpg "Western Blot: IZUMO1 Antibody [NBP1-83086]")
Western Blot: IZUMO1 Antibody [NBP1-83086]
Western Blot: IZUMO1 Antibody [NBP1-83086] - Protein extracts from four different pig ejaculates and a human sample. Lanes F: fresh sperm. Lanes F-T: frozen-thawed sperm. Lanes H: human fresh sperm. Image submitted by a verified customer review.![Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]](https://resources.bio-techne.com/images/products/IZUMO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83086-img0015.jpg "Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]")
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]](https://resources.bio-techne.com/images/products/IZUMO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83086-img0013.jpg "Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]")
![Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]](https://resources.bio-techne.com/images/products/IZUMO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83086-img0014.jpg "Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]")
![Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]](https://resources.bio-techne.com/images/products/IZUMO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83086-img0016.jpg "Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]")
Applications for IZUMO1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunocytochemistry reported in scientific literature (PMID:31391940). IZUMO1 antibody validated for WB from a verified customer review.
Reviewed Applications
Read 1 review rated 5 using NBP1-83086 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IZUMO1
Long Name
Izumo Sperm-egg Fusion 1
Alternate Names
OBF
Gene Symbol
IZUMO1
Additional IZUMO1 Products
Product Documents for IZUMO1 Antibody - BSA Free
Product Specific Notices for IZUMO1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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