Islet-1 Antibody (23GB6115)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50460

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NBP3-50460-100ul
NBP3-50460-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6115

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Islet-1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Islet-1 (Uniprot: P61371)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Islet-1 Antibody (23GB6115)

Islet-1 Antibody (23GB6115)

Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody (23GB6115)[NBP3-50460] -

Immunocytochemical staining of HAP-1 cells with Islet-1 antibody (1:1,000). Nuclei were stained blue with DAPI; Islet-1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Islet-1 Antibody (23GB6115)

Flow Cytometry: Islet-1 Antibody (23GB6115)[NBP3-50460] -

Flow cytometric analysis of Islet-1expression in HAP-1 cells using Islet-1 antibody (1:2,000). Green, isotype control; red, ISL1.
Islet-1 Antibody (23GB6115)

Western Blot: Islet-1 Antibody (23GB6115)[NBP3-50460] -

Western blotting analysis using Islet-1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Islet-1 Antibody (23GB6115)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Islet-1

Islet 1 encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Alternate Names

ISL1, Islet1

Gene Symbol

ISL1

Additional Islet-1 Products

Product Documents for Islet-1 Antibody (23GB6115)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Islet-1 Antibody (23GB6115)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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