Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44056
![Western Blot: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056]](https://resources.bio-techne.com/images/products/Iododeoxyuridine-IdU-Antibody-32D8-D9-Western-Blot-NBP2-44056-img0003.jpg "Western Blot: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056]")
Discontinued Product
NBP2-44056 has been discontinued.
View all Iododeoxyuridine/IdU products.
Key Product Details
Species Reactivity
Validated:
Human, Non-species specific
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 32D8.D9
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Iododeoxyuridine/Iododeoxyuridine/IdU antibody (32D8.D9) was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Reactivity Notes
Cross-reactivity is not observed with BrdU, CldU or FdU.
Specificity
This antibody is specific for Iododeoxyuridine/IdU. Cross-reactivity is not observed with BrdU, CldU or FdU.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This antibody Monoclonal Antibody was purified from ascites fluid by Protein A chromatography
Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free
Western Blot: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056]
Western Blot: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056] - Western Blot of Iododeoxyuridine/Iododeoxyuridine/IdU antibody (32D8.D9). Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: Iododeoxyuridine/IdU. Lane 5: CldU. Load: 20 ug per lane.Primary antibody: Anti-Iododeoxyuridine/IdU antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed: Iododeoxyuridine/IdU. Other band(s): no cross reactive bands were observed for other nucleoside analogs.
N/A: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056]
Applications for Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:10000
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Western Blot
1:2000-1:5000
Application Notes
This product has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated IdU thymidine analog from replicated cells.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Iododeoxyuridine/IdU
Alternate Names
2'-Deoxy-5-iodouridine, 54-42-2, 5-Iodo-2'-deoxyuridine, 5-Iododeoxyuridine, Idoxuridin, Iododeoxyridine, Iodoxuridine, Joddeoxiuridin
Product Documents for Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free
Product Specific Notices for Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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