Insulin R/CD220 Antibody (23GB2050)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50531
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2050
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Insulin R/CD220 (Uniprot: P06213)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Insulin R/CD220 Antibody (23GB2050)
Immunocytochemistry/ Immunofluorescence: Insulin R/CD220 Antibody (23GB2050)[NBP3-50531] -
Immunocytochemical staining of HepG2 cells with Insulin R/CD220 antibody (1:1,000). Nuclei were stained blue with DAPI; Insulin receptor was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: Insulin R/CD220 Antibody (23GB2050)[NBP3-50531] -
Flow cytometric analysis of Insulin R/CD220expression in HT-1080 cells using Insulin R/CD220 antibody (1:2,000). Green, isotype control; red, Insulin receptor.
Western Blot: Insulin R/CD220 Antibody (23GB2050)[NBP3-50531] -
Western blotting analysis using Insulin R/CD220 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for Insulin R/CD220 Antibody (23GB2050)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Insulin R/CD220
Long Name
Insulin Receptor
Alternate Names
CD220, INSR, InsulinR
Gene Symbol
INSR
Additional Insulin R/CD220 Products
Product Documents for Insulin R/CD220 Antibody (23GB2050)
Product Specific Notices for Insulin R/CD220 Antibody (23GB2050)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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