Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-5 R alpha/CD125 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑5 R alpha/CD125

Specificity

Detects human IL-5 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IL‑1 RII, rhIL-2 R beta, rhIL-2 R gamma, rhIL-3 R is observed and less than 1% cross-reactivity with rhIL‑1 RI, rhIL‑4 R, rhIL‑6 R, rhIL‑7 R, rhIL‑9 R, and rhIL‑10 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for IL-5R alpha/CD125 Antibody [PE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-5 R alpha/CD125

The protein encoded by the IL5RA gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. (provided by RefSeq)

Long Name

Interleukin 5 Receptor alpha

Alternate Names

CD125, IL-5Ra, IL5R alpha, IL5RA

Gene Symbol

IL5RA

Additional IL-5 R alpha/CD125 Products

Product Documents for IL-5R alpha/CD125 Antibody [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-5R alpha/CD125 Antibody [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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