Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Biotin

Antibody Source

Monoclonal Human IgG1 Clone # Hu212

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-5 Antibodies »

Product Specifications

Immunogen

Human IL-5

Specificity

Detects human IL-5 based on Mepolizumab therapeutic antibody.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for IL-5 Antibody (Hu212) [Biotin]

Detection of IL-5 in Human PBMCs by Flow Cytometry.

Human PBMCs (A) treated with 50 ng/mL PMA and 1 ug/mL Calcium Ionomycin for 6 hours or (B) resting were stained with Human Anti-Human IL-5 (Mepolizumab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB11433B) followed by APC-conjugated Streptavidin (F0050) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for IL-5 Antibody (Hu212) [Biotin]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-5

IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Additional IL-5 Products

Product Documents for IL-5 Antibody (Hu212) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-5 Antibody (Hu212) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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