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IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00468

Novus Biologicals, part of Bio-Techne
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NBP2-00468-100Tests
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PerCP/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H6

Concentration

5 mg/ml

Product Specifications

Immunogen

Tissue / cell preparation. IL3R transfected COS cells.

Localization

Type I membrane protein.

Specificity

IL3RA (6H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5] [NBP2-00468] - Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) Alexa Fluor 647 and Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (NBP1-42839) (left) or Anti-Human CD123 PerCP-Cy5.5 (right). Cells in the monocyte gate were used for analysis.

Applications for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-3R alpha

The 6H6 monoclonal antibody reacts with human CD123, the a chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Gene Symbol

IL3RA

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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