IL-32 alpha Antibody (12) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06577
![Western Blot: IL-32 alpha Antibody (12) [NBP3-06577]](https://resources.bio-techne.com/images/products/IL-32-alpha-Antibody-12-Western-Blot-NBP3-06577-img0001.jpg "Western Blot: IL-32 alpha Antibody (12) [NBP3-06577]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 12
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-32 alpha (Accession#: NP_001012651.1; Met1-Lys131).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for IL-32 alpha Antibody (12) - Azide and BSA Free
Western Blot: IL-32 alpha Antibody (12) [NBP3-06577]
Western Blot: IL-32 alpha Antibody (12) [NBP3-06577] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.)Applications for IL-32 alpha Antibody (12) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-32 alpha
IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN-gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression. No ortholog has been found in mouse.
Long Name
Interleukin 32 alpha
Alternate Names
IL32 alpha
Gene Symbol
IL32
Additional IL-32 alpha Products
Product Documents for IL-32 alpha Antibody (12) - Azide and BSA Free
Product Specific Notices for IL-32 alpha Antibody (12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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