IL-11R alpha Antibody (10Z3S9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16809
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10Z3S9 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 323-422 of human IL-11R alpha (Q14626). EIPAWGQLHTQPEVEPQVDSPAPPRPSLQPHPRLLDHRDSVEQVAVLASLGILSFLGLVAGALALGLWLRLRRGGKDGSPKPGFLASVIPVDRRPGAPNL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit IL-11R alpha Antibody (10Z3S9) (NBP3-16809) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for IL-11R alpha Antibody (10Z3S9)
![Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809]](https://resources.bio-techne.com/images/products/IL-11R-alpha-Antibody-10Z3S9-Western-Blot-NBP3-16809-img0008.jpg "Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809]")
Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809]
Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Western blot analysis of extracts of various cell lines, using (NBP3-16809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.![Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809]](https://resources.bio-techne.com/images/products/IL-11R-alpha-Antibody-10Z3S9-Western-Blot-NBP3-16809-img0009.jpg "Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809]")
Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809]
Western Blot: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Western blot analysis of extracts of Rat brain, using (NBP3-16809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] -
Immunocytochemistry/ Immunofluorescence: IL-11R alpha Antibody (10Z3S9) [NBP3-16809] - Confocal imaging of K-562 cells using IL-11R alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.
Applications for IL-11R alpha Antibody (10Z3S9)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IL-11 R alpha
IL-11 Ra (Interleukin-11 receptor alpha) is a widely expressed transmembrane protein that associates with gp130 to form a functional receptor complex for the cytokine IL-11. gp130 also functions as a subunit in the receptors for Cardiotrophin-1, CNTF, IL-6, IL-27, IL-31, LIF, and Oncostatin M. IL-11 Ra plays a role in female reproduction and the survival of oligodendrocytes and colonic epithelial cells. It enhances osteoclast differentiation and bone remodeling but inhibits adipocyte differentiation. Soluble IL-11 Ra confers IL-11 responsiveness to cells expressing gp130, while in cells expressing transmembrane IL-11 Ra and gp130, soluble IL-11 Ra acts as an IL-11 antagonist.
Long Name
Interleukin 11 Receptor alpha
Alternate Names
IL-11Ra, IL11R alpha, IL11RA
Gene Symbol
IL11RA
Additional IL-11 R alpha Products
Product Documents for IL-11R alpha Antibody (10Z3S9)
Product Specific Notices for IL-11R alpha Antibody (10Z3S9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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