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IgA2 Antibody (RM125) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62025B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-62025B

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM125 expressed in HEK293

Concentration

1 mg/ml

Product Specifications

Immunogen

Human IgA2

Specificity

This antibody reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgA2 Antibody (RM125) [Biotin]

IgA2 Antibody (RM125) [Biotin]

ELISA: IgA2 Antibody (RM125) - Azide and BSA Free [NBP2-62025B] -

ELISA: IgA2 Antibody (RM125) - Azide and BSA Free [NBP2-62025B] - ELISA of human immunoglobulins shows RM125 only reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgA2 Antibody (RM125) [Biotin]

ELISA: IgA2 Antibody (RM125) - Azide and BSA Free [NBP2-62025B] -

ELISA: IgA2 Antibody (RM125) - Azide and BSA Free [NBP2-62025B] - A titer ELISA using RM125. The plate was coated with different amounts of human IgA2. A serial dilution of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgA2 Antibody (RM125) [Biotin]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5ug/ml - 2ug/ml

Immunohistochemistry

0.5ug/ml - 2ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgA2

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with human IgA2 from myeloma patient serum.

Alternate Names

immunoglobulin heavy constant alpha 2 (A2m marker)

Gene Symbol

IGHA2

Additional IgA2 Products

Product Documents for IgA2 Antibody (RM125) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IgA2 Antibody (RM125) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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