ID1 Antibody (CL11995) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44357

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL11995

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

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Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P41134, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ID1 Antibody (CL11995) - Azide and BSA Free

ID1 Antibody (CL11995) - Azide and BSA Free Immunohistochemistry-Paraffin: ID1 Antibody [NBP3-44357]

Immunohistochemistry-Paraffin: ID1 Antibody [NBP3-44357]

Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.
ID1 Antibody (CL11995) - Azide and BSA Free Immunohistochemistry-Paraffin: ID1 Antibody [NBP3-44357]

Immunohistochemistry-Paraffin: ID1 Antibody [NBP3-44357]

Analysis in human seminal vesicle and liver tissues using NBP3-44357 antibody. Corresponding ID1 RNA-seq data are presented for the same tissues.
ID1 Antibody (CL11995) - Azide and BSA Free Immunohistochemistry-Paraffin: ID1 Antibody [NBP3-44357]

Immunohistochemistry-Paraffin: ID1 Antibody [NBP3-44357]

Staining of human colon shows moderate to strong cytoplasmic and nuclear positivity in glandular cells.
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Applications for ID1 Antibody (CL11995) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ID1

Id1 is encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Inhibitor of DNA Binding 1

Alternate Names

BHLHB24, bHLHb24DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, dJ857M17.1.2, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein), ID, inhibitor of differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Gene Symbol

ID1

Additional ID1 Products

Product Documents for ID1 Antibody (CL11995) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ID1 Antibody (CL11995) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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