Human Wnt-7b Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3460
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Wnt-7b
Leu32-Glu72 & Thr216-Ala283
Accession # P56706
Leu32-Glu72 & Thr216-Ala283
Accession # P56706
Specificity
Detects human Wnt-7b in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Wnt-7b Antibody
Detection of Wnt-7b in Human Breast Cancer
Wnt-7b was detected in immersion fixed paraffin-embedded sections of human breast cancer using Goat Anti-Human Wnt-7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Wnt-7b Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue and human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue and human breast cancer tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-7b
Long Name
Wingless-type MMTV Integration Site Family, Member 7B
Alternate Names
Wnt7b
Gene Symbol
WNT7B
UniProt
Additional Wnt-7b Products
Product Documents for Human Wnt-7b Antibody
Product Specific Notices for Human Wnt-7b Antibody
For research use only
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