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Human VISTA/B7-H5/PD-1H Antibody

Bio-Techne includes R&D Systems | Catalog # AF7126

Catalog #
Size / Price

Key Product Details

Species Reactivity






CyTOF-ready, ELISA, Flow Cytometry, Western Blot



Antibody Source

Polyclonal Sheep IgG

Product Summary for Human VISTA/B7-H5/PD-1H Antibody


Mouse myeloma cell line NS0-derived recombinant human VISTA/B7-H5/PD-1H
Accession # Q9H7M9


Detects human VISTA/B7-H5/PD-1H in direct ELISAs and Western blots.







Scientific Data Images for Human VISTA/B7-H5/PD-1H Antibody

Detection of Human VISTA/B7-H5/PD-1H antibody by Western Blot.

Detection of Human VISTA/B7-H5/PD-1H by Western Blot.

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human VISTA/B7-H5/PD-1H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7126) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VISTA/B7-H5/PD-1H at approximately 45-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of VISTA/B7-H5/PD-1H antibody in Human PBMCs antibody by Flow Cytometry.

Detection of VISTA/B7-H5/PD-1H in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD11b/Integrin alpha M APC-conjugated Monoclonal Antibody (Catalog # FAB16991A) and either (A) Sheep Anti-Human VISTA/B7-H5/PD-1H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7126) or (B) Normal Sheep IgG Control (Catalog # 5-001-A) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). View our protocol for Staining Membrane-associated Proteins.
Human VISTA/B7-H5/PD-1H Antibody in ELISA Standard Curve.

Human VISTA/B7-H5/PD-1H ELISA Standard Curve.

Recombinant Human VISTA/B7-H5/PD-1H protein was serially diluted 2-fold and captured by Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human VISTA/B7-H5/PD-1H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7126) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human VISTA/B7-H5/PD-1H Antibody

Recommended Usage


Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.


This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71264).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human VISTA/B7-H5/PD-1H DuoSet ELISA Kit (Catalog # DY7126-05) for convenient development of a sandwich ELISA.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Western Blot

1 µg/mL
Sample: Human placenta tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF7126 in the following applications:

Formulation, Preparation, and Storage


Antigen Affinity-purified


Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, VISTA appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, VISTA both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human VISTA contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human VISTA undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human VISTA shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol



Additional VISTA/B7-H5/PD-1H Products

Product Documents for Human VISTA/B7-H5/PD-1H Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VISTA/B7-H5/PD-1H Antibody

For research use only