Twist-2 was detected in immersion fixed A549 human lung carcinoma cells treated with Recombinant Human TGF-beta 1 (Catalog # 240-B) using Human Twist-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Twist-2 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line treated with Recombinant Human TGF-beta 1 (Catalog # 240-B)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Twist-2

Twist-2 (Twist-related protein 2; also Dermo-1 and bHLHa39) is a 19-20 kDa Class B member of the bHLH transcription factor family of proteins. It is expressed in embryonic mesodermal and ectodermal derivatives, and is widely expressed in adult cells, including preosteoblasts, gastric chief cells, preadipocytes and myeloid progenitor cells. Twist-2 is a transcriptional repressor that heterodimerizes with multiple partners such as E12, SREBP1 and MEF2. Human Twist-2 is 160 amino acids (aa) in length. It contains a bHLH domain (aa 65-122) with an embedded DNA-binding motif. Full length human Twist-2 shares 82% and 99% aa identity with human Twist-1 and mouse Twist-2, respectively.

Long Name

Twist Related Protein 2 [Drosophila]

Alternate Names

bHLHa39, DERMO1, Twist2

Entrez Gene IDs

117581 (Human)

Gene Symbol

TWIST2

UniProt

Q8WVJ9 (Human)

Additional Twist-2 Products

Product Documents for Human Twist-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Twist-2 Antibody

For research use only

Citations
Reviews

Human Twist-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6249

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Twist-2 Antibody

Twist‑2 in A549 Human Cell Line.