Detects human Trappin-2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM‑2, and human Neutrophil Elastase is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Trappin‑2/Elafin Biotinylated Antibody

Human Trappin-2 / Elafin ELISA Standard Curve

Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI) was serially diluted and captured by Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Trappin-2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Trappin‑2/Elafin Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI)

Human Trappin-2/Elafin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human Trappin-2/Elafin Antibody (Catalog # AF1747)
Standard: Recombinant Human Trappin-2/Elafin Protein, CF (Catalog # 1747-PI)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF1747 in the following applications:

Luminex Development (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trappin-2/Elafin

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72-117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally.

References

Schalkwijk, J. et al. (1999) Biochem. J. 340:569.

Long Name

Elastase Inhibitor, Transglutaminase Substrate, Serine Protease

Alternate Names

Elafin, ESI, PI3, SKALP, Trappin2

Entrez Gene IDs

5266 (Human)

Gene Symbol

PI3

UniProt

P19957

Additional Trappin-2/Elafin Products

Product Documents for Human Trappin‑2/Elafin Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Trappin‑2/Elafin Biotinylated Antibody

For research use only

Citations
Reviews

Human Trappin‑2/Elafin Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1747

Key Product Details

Species Reactivity

Validated:

Cited:

Applications