Human TNF RI/TNFRSF1A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6251
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 925224
Product Specifications
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Arg235-Arg455
Accession # P19438
Arg235-Arg455
Accession # P19438
Specificity
Detects human TNF RI/TNFRSF1A in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human TNF RI/TNFRSF1A Antibody
Detection of Human TNF RI/TNFRSF1A by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB6251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TNF RI/TNFRSF1A by Western Blot
NPS-1034 activates GADD45A, inhibits the MET and TNFRSF1A downstream signaling pathway proteins’ expression level, and upregulates the pro-apoptosis protein expression level in RCC cells. (A) Validation of NPS-1034, an MET/AXL inhibitor, on the MET downstream pathway protein expression level in RCC cells. (B) Assessment of the regulatory role of GADD45A and TNFR1-related pathways, such as the I kappaB alpha/NF kappaB signaling pathway. (C) The expression levels of several pro-apoptosis proteins were detected in RCC cells treated with NPS-1034 (0, 20, and 80 μM) for 48 h via Western blotting. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39451232), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TNF RI/TNFRSF1A by Western Blot
The high expression of GADD45A correlates with a favorable prognosis and effect on the survival rate of patients with RCC. TNFRSF1A overexpression reversed the anticancer effects of NPS-1034 in RCC cells. (A) A volcano plot showing the differential expression of genes altered by NPS-1034 treatment in RCC cells. (B) Relative GADD45A mRNA expression in 877 patients. Kaplan–Meier plots showing the survival probabilities of RCC patients stratified by GADD45A expression. Patient survival details were derived from TCGA. (C) The increases in GADD45A-related pathway expression and the decreases in I kappaB alpha/NF kappaB-related pathway expression are all significantly reversed via TNFRSF1A overexpression. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39451232), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human TNF RI/TNFRSF1A Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line
Sample: A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A
Long Name
Tumor Necrosis Factor Receptor I
Alternate Names
CD120a, TNFRI, TNFRSF1A
Gene Symbol
TNFRSF1A
UniProt
Additional TNF RI/TNFRSF1A Products
Product Documents for Human TNF RI/TNFRSF1A Antibody
Product Specific Notices for Human TNF RI/TNFRSF1A Antibody
For research use only
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